FIGURE

Fig. 1

ID

ZDB-FIG-140422-69

Publication

Superina et al., 2014 - Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

MZjek mutant embryos lack extracellular heparan sulfate proteoglycans and display multiple morphological abnormalities. (A,B) Representative confocal micrographs of anti- heparan sulfate PG staining in the animal pole of shield-staged wildtype (A) and MZjek mutant (B) embryos (N=20,20 respectively). Anti-heparan sulfate staining is clearly present in the extracellular space of wildtype embryos. In MZjek, there is an absence of HS staining, suggesting HS GAGs are not synthesized in the mutant. (C,D) Live images of 48 hpf WT (C) and MZjek mutant embryos (D) mounted for lateral, dorsal and ventral views. MZjek (D) exhibit an expanded mediolateral axis (bars), defects in axial extension, brain patterning defects (arrowheads) and disrupted heart morphogenesis.

Expression Data

Antibody:	Ab-10E4
Fish:	WT ugdh^m310/m310
Anatomical Term:	anatomical structure extracellular space
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ugdh^m310/m310
Observed In:	caudal fin central nervous system central nervous system development heart heart development whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 387(2), Superina, S., Borovina, A., and Ciruna, B., Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry, 154-166, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.