FIGURE

Fig. 5

ID

ZDB-FIG-140115-21

Publication

Kim et al., 2013 - A Complex of BBS1 and NPHP7 Is Required for Cilia Motility in Zebrafish

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Fig. 5

Depletion of zBbs1 and zNphp7.2 caused defects in heart looping.

To examine organ laterality defects, zebrafish embryos were examined for changes in heart looping. (A) In situ hybridisation (myosin light chain 7; myl7) of both zbbs1 and znphp7.2 morphant embryos at 55 hpf showed defective heart looping. (heart looping to the right (R = normal), without heart looping (mid) and looping to the left (L = inversed)) (B) Whereas the hearts of control embryos showed more than 90% rightward looping, approximately 50% of zBbs1-depleted embryos and (C) 35–75% of zNphp7.2-depleted embryos had situs inversus or mid position of the heart. The numbers in the brackets (n) are the numbers of total embryos which were examined.

Expression Data

Gene:	myl7
Fish:	WT WT + MO1-glis2b WT + MO2-bbs1
Knockdown Reagents:	MO1-glis2b MO2-bbs1
Anatomical Term:	heart
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-glis2b WT + MO2-bbs1
Knockdown Reagents:	MO1-glis2b MO2-bbs1
Observed In:	determination of left/right symmetry heart looping
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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