FIGURE

Fig. 3

ID

ZDB-FIG-140115-19

Publication

Kim et al., 2013 - A Complex of BBS1 and NPHP7 Is Required for Cilia Motility in Zebrafish

Other Figures

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Fig. 3

Depletion of zBbs1 and zNphp7.2 causes dose-dependent hydrocephalus and pronephric cysts.

Zebrafish embryos injected with MOs at the 1-cell stage were evaluated for their phenotype at 55 hpf. (A) Control zebrafish embryo with magnified normal brain area. Scale bar = 500 μm. (B and C) zbbs1 AUG MO-injected embryo and znphp7.2 SP1 MO-injected embryo with hydrocephalus. (D) The Tg(WT1b:EGFP) transgenic line shows normal pronephric glomerulus and tubules (Scale bar = 100 μm), (E and F) zbbs1 AUG MO-injected embryo and znphp7.2 SP1 MO-injected embryo show pronephric cysts (asterisks). At 55 hpf, larval dysmorphy was categorized according to the visual scale (Fig. S3). The degree of dysmorphy was increased with the amount of injected MOs (G, zbbs1 AUG MO; H, znphp7.2 SP1 MO). (G and H) Cystic pronephric phenotypes were dose-dependent in zebrafish embryos injected with zbbs1 and znphp7.2 MO respectively. The graphs show percentages of the number (n) of embryos that were examined.

Expression Data

Gene:	EGFP
Fish:	li1Tg li1Tg + MO1-glis2b li1Tg + MO2-bbs1
Knockdown Reagents:	MO1-glis2b MO2-bbs1
Anatomical Terms:	pronephric glomerulus pronephric tubule
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-glis2b WT + MO2-bbs1 li1Tg + MO1-glis2b li1Tg + MO2-bbs1
Knockdown Reagents:	MO1-glis2b MO2-bbs1
Observed In:	brain pronephros trunk
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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