FIGURE

Fig. 1

ID

ZDB-FIG-130625-39

Publication

Schmid et al., 2013 - Loss of ALS-associated TDP-43 in zebrafish causes muscle degeneration, vascular dysfunction, and reduced motor neuron axon outgrowth

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Fig. 1

Overview of human TDP-43 and zebrafish orthologs and Western blot of their loss-of-function alleles. (A) Schematic representation of human TDP-43 (turquois), zebrafish Tardbp (red), and zebrafish Tardbpl protein (orange); blue bars represent nuclear localization sequence; green bars represent nuclear export sequence; RRM, RNA recognition motif. (B, Top) Tardbp specific monoclonal antibody 4A12-111 detects the 43-kDa Tardbp protein in adult brain from wild-type and tardbpl^mde222-/- but not from tardbp^mde198-/- and tardbp^mde897-/- fish. Asterisk marks an unspecific band. (Middle) Tardbpl-specific monoclonal antibody 5F5-11 detects the approximately 34-kDa Tardbpl protein in adult brain from wild-type, tardbp^mde198-/- and tardbp^mde897-/- but not from tardbpl^mde222-/- fish. (Bottom) α-Tubulin serves as a loading control.

Expression Data

Genes:	tardbpa tardbpb
Antibodies:	Ab1-tardbpl Ab5-tardbp
Fish:	AB tardbpa^{mde222/mde222} (AB) tardbpb^{mde198/mde198} (AB) tardbpb^{mde897/mde897} (AB)
Anatomical Term:	brain
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tardbpa^{mde222/mde222} (AB) tardbpb^{mde198/mde198} (AB) tardbpb^{mde897/mde897} (AB)
Observed In:	brain
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA