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Inhibition of Alk activity by CEP-26939 phenocopies alk morphant defects. (A,C,E,G,I) Embryos at 60 hpf treated with 1% DMSO from 24 hpf were used as negative controls. (B,D,F,H,J) 60 hpf embryos treated with 40 μM CEP-26939 from 24 hpf. (A,B) Inhibitor treated embryos showed more TUNEL-positive apoptotic cells in mid- and hindbrain (B) when compared to DMSO treated embryos (A). (C,D) No significant change was observed for her6 expression in inhibitor treated embryos. (E,F) neurog1 was only slightly reduced in inhibitor treated (F) compared to DMSO treated embryos (E). (G,H,I,J) Both neurod4 and ascl1b expression domains in mid- and hindbrain were almost completely absent in inhibitor treated embryos (H,J). All images show dorsal views with the focal plane on mid- and hindbrain, with anterior to the left. Numbers in each image indicate individuals similar as that shown as representatives and total numbers of embryos investigated. Scale bars: 100 μm.
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