FIGURE

Fig. S2

ID

ZDB-FIG-130618-35

Publication

Yao et al., 2013 - Anaplastic lymphoma kinase is required for neurogenesis in the developing central nervous system of zebrafish

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Fig. S2

Heat-shock inducible transgenic zebrafish lines. (A,B) Diagrams illustrating transgenic cassettes for control line (A) and alk overexpression lines (B). The construct reported by Bajoghli et al. contains eight heat shock element repeats (HSE) with bidirectional promoter activity. This construct was engineered in such a way that alk and cfp were put on each side (B), or just cfp in the control line (A). (C?E) 24hpf embryos from the control line (HSE:cfp, C), alk overexpression line 1 (alk:HSE:cfp¹, D), and independent alk overexpression line 2 (alk:HSE:cfp², E). All embryos received a 1.5 hour long heat shock at 39.5�C and at 10.5 hpf. Bright field images on top and images of the same embryos in CFP channel below. Inserts show representative transgene positive embryos in each line. Arrows point to transgenic negative siblings in each line. They are morphologically normal and not visible in the CFP channel.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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