FIGURE

Fig. 7

ID

ZDB-FIG-130416-26

Publication

Navis et al., 2013 - Cftr controls lumen expansion and function of Kupffer's vesicle in zebrafish

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Fig. 7

Fluid secretion regulates KV size. (A,B) DIC imaging of KV (arrowheads) in (A) DMSO control and (B) 10 μM forskolin-, 40 μM IBMX-treated embryos. (C) The KV area of DMSO and treated fish is quantified. DMSO n=15; forskolin + IBMX, n=13; ^*P<0.01. (D,E) DIC imaging of KV (arrowheads) in (D) DMSO control and (E) 1 μM ouabain-treated embryos at 10 ss. (F) Quantification of KV area in DMSO-and ouabain-treated embryos. DMSO, n=10; ouabain, n=10; ^*P<0.02. (G-I) Live confocal imaging of Tg(sox17:GFP)-expressing fish treated with (G) DMSO, (H) ouabain or (I) forskolin and IBMX. Error bars represent s.e.m. Scale bars: 50 μm.

Expression Data

Gene:	GFP
Fish:	s870Tg
Conditions:	chemical treatment: 3-isobutyl-1-methylxanthine chemical treatment: ouabain
Anatomical Term:	Kupffer's vesicle
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT s870Tg
Conditions:	chemical treatment: 3-isobutyl-1-methylxanthine chemical treatment: ouabain
Observed In:	Kupffer's vesicle P-type sodium:potassium-exchanging transporter activity protein kinase A signaling
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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