FIGURE

Fig. 4

ID

ZDB-FIG-130416-23

Publication

Navis et al., 2013 - Cftr controls lumen expansion and function of Kupffer's vesicle in zebrafish

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Fig. 4

Apical-basal polarity is not affected in cftr^pd1049 mutants. (A,B) Confocal image and associated orthogonal projections of WT and cftr^pd1049 mutant fish stained for ZO-1 and Cadherin. (A′-A′′ ′,B′-B′′ ′) Magnification of the KV epithelium to show localization of the polarity markers. (C,D) Confocal image and associated orthogonal projections of WT and cftr^pd1049 mutant fish stained for aPKC and expressing Tg(sox17:GFP). (E,F) Confocal image of WT and cftr^pd1049 mutant KV expressing apically localized, GFP-tagged Podocalyxin. (G,H) Live confocal images of WT and cftr^pd1049 mutant fish co-expressing an apical marker, RFP-Clic5b, and Tg(sox17:GFP). Scale bars: 50 μm.

Expression Data

Gene:	GFP
Antibody:	Ab1-prkca
Fish:	pd1080Tg s870Tg cftr^{pd1049/pd1049}; pd1080Tg cftr^{pd1049/pd1049}; s870Tg
Condition:	heat shock
Anatomical Terms:	Kupffer's vesicle Kupffer's vesicle apical plasma membrane
Stage Range:	1-4 somites to 5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pd1080Tg cftr^{pd1049/pd1049}; pd1080Tg cftr^{pd1049/pd1049}; s870Tg
Condition:	heat shock
Observed In:	Kupffer's vesicle establishment of epithelial cell apical/basal polarity
Stage Range:	1-4 somites to 5-9 somites

Phenotype Detail

Acknowledgments

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