Fig. 2

Organ laterality is disrupted in cftr¹⁰⁴⁹ mutant embryos. Heterozygous cftr^pd1049 fish were mated to assess organ laterality. (A) Ventral view of 4 days post-fertilization (dpf) WT and cftr^pd1049 mutant larvae expressing dsRed in the liver (arrows). (B) Quantification of liver orientation in WT and cftr^pd1049 mutants. Liver orientation is reversed in 27% of homozygous mutants. WT, n=103; cftr^pd1049, n=30. (C) Ventral view of representative cftr^pd1049 mutants showing cmlc2 expression pattern. (D) Quantification of heart looping in WT and cftr^pd1049 mutants. WT, n=408; cftr^pd1049, n=138. (E) Dorsal view of cftr^pd1049 mutant embryos displaying left, right, left>right, right>left and left=right spaw expression patterns. (F) Quantification of spaw expression in WT and cftr^pd1049 mutants. WT, n=146; cftr^pd1049, n=73.