FIGURE

Fig. 2

ID

ZDB-FIG-130411-20

Publication

Gibbs et al., 2013 - Two dynamin-2 genes are required for normal zebrafish development

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Fig. 2

Structure and expression of dnm2 and dnm2-like.(A) Molecular intron-exon organization of human DNM2, zebrafish dnm2 and zebrafish dnm2-like. (B) Protein structure of zebrafish Dnm2 and Dnm2-like compared to human DNM2. Percent identity between zebrafish and human protein domains was calculated using BLASTP. PH, pleckstrin homology domain; GED, GTPase effector domain; PRD, proline-rich domain. (C) RT-PCR was used to assay spatial expression levels of dnm2 and dnm2-like in tissues isolated from adult zebrafish. Primers for ef1α were used as an internal control. (D) RT-PCR was used to assay temporal expression levels of dnm2 and dnm2-like between 0 hpf and 24 hpf. All classical dynamins appear to be deposited as maternal mRNAs and expressed throughout early development.

Expression Data

Genes:	dnm2a dnm2b dnm3a
Fish:	AB
Anatomical Terms:	brain heart liver muscle spinal cord whole organism
Stage Range:	1-cell to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-dnm2b AB + MO2-dnm2a
Knockdown Reagents:	MO1-dnm2b MO2-dnm2a
Observed In:	eye muscle pericardium pigmentation post-vent region somite whole organism whole organism anterior-posterior axis yolk
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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