Fig. 9

kctd12.2 expression is low in both habenulae when the epthalamus is divided. Embryos were processed as in Fig. 8 but assayed instead for kctd12.2 expression. ((A)–(C)) kctd12.2 expression is higher on the right in embryos with an oval shaped pineal. (D) kctd12.2 is expressed at undetectable levels in the majority of embryos with divided pineal organs. The low or undetectable levels of kctd12.2 expression were not due to technical difficulties, as WT embryos processed in parallel had normal levels of expression. Closed arrowheads indicate the habenulae with higher level of kctd12.2 expression. Open arrowheads indicate the habenulae with lower expression. (E) The distribution of kctd12.2 expression pattern in each group of embryos. “Other” includes embryos with either a single kctd12.2 expression domain or many small areas of kctd12.2 expression. ne17 for each group. The experiment was repeated three times and representative images are shown. All images are dorsal views, anterior to the top. Scale bar=50 μm.