FIGURE

Fig. 10

ID

ZDB-FIG-130123-64

Publication

Schweitzer et al., 2013 - Sim1a and Arnt2 contribute to hypothalamo-spinal axon guidance by regulating Robo2 activity via a Robo3-dependent mechanism

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Fig. 10

The N-terminal domain present in Robo3A isoforms is important for interfering with Robo2-dependent repulsion. Dorsal views of hindbrain confocal z-projections of 72 hpf embryos. Anterior is towards the left. Embryos were co-injected with hcrt:gal4 and UAS constructs indicated on the left. (A,B) Hcrt neurons form tdTomato-positive longitudinal axons (A, arrows), which project laterally (B, arrows) to the MLF (B, arrowheads). (C,D) After mis-expression of robo3a.1 in Hcrt neurons, tdTomato-positive longitudinal axons are medially displaced towards the midline (C,D, arrows) and project together with the medial longitudinal fascicle (D, arrowheads). Small arrows indicate ectopic midline crossing events (C). (E,F) Upon mis-expression of robo3b.1 in Hcrt neurons, tdTomato-positive longitudinal axons project normally (E,F, arrows). (G-J) After mis-expression of mouse Robo3A.1 (G,H) or Robo3A.2 (I,J) in Hcrt neurons, tdTomato-positive longitudinal axons are displaced towards the midline (G-J, arrows) and project together with the medial longitudinal fascicle (H,J, arrowheads). Small arrow in H indicates midline crossing. (K) Quantification. Asterisks in A,C,E,G,I indicate Hcrt neurons, asterisks in B,D,F,H,J indicate nucMLF neurons. Scale bar: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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