FIGURE

Fig. 7

ID

ZDB-FIG-130123-60

Publication

Schweitzer et al., 2013 - Sim1a and Arnt2 contribute to hypothalamo-spinal axon guidance by regulating Robo2 activity via a Robo3-dependent mechanism

Other Figures

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Fig. 7

Dcc-mediated attraction causes medial displacement of otpb:gfp longitudinal axons after sim1a knockdown. Dorsal views of hindbrain confocal z-projections of 48 hpf otpb:gfp embryos. Anterior is towards the left. (A-D) GFP-positive longitudinal axons are medially displaced towards the midline (A, arrows) after co-injection of sim1a MO and control MO in otpb:gfp embryos. Medial displacement of longitudinal otpb:gfp axons (C, arrows) is reduced after combined injection of sim1a MO and dcc MO. (E) Quantification of the distance between otpb:gfp-positive longitudinal axons (I) or of the distance between Mauthner neurons (II) of indicated treatments. Arrowheads in B,D indicate normal midline crossing of MA neurons. ^*P<0.001; n.s., not significant. Scale bar: 50 μm.

Expression Data

Gene:	EGFP
Antibody:	Ab-3A10
Fish:	zc49Tg + MO2-sim1a zc49Tg + MO2-dcc + MO2-sim1a
Knockdown Reagents:	MO2-dcc MO2-sim1a
Anatomical Terms:	diencephalic nucleus axon Mauthner neuron Mauthner neuron axon
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zc49Tg + MO2-sim1a
Knockdown Reagent:	MO2-sim1a
Observed In:	diencephalic nucleus axon hindbrain medial region
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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