Fig. 9

Overexpression of robo3a.1 leads to increased medial displacement of longitudinal HTS axons in ast^te284 but not in ast^ti272z embryos. Dorsal views of confocal z-projections of the brain and hindbrain of 72 hpf hcrt:tdtomato embryos. Anterior is towards the left. (A-H) Medial displacement phenotype of hcrt:tdtomato HTS axons is similar in ast^{ti272z/ti272z} and ast^{ti272z/ti272z}; hsp70l:robo3a.1 embryos after heat-shock treatment. (J-Q) Medial displacement phenotype of hcrt:tdtomato HTS axons is increased in ast^te284/te284; hsp70l:robo3a.1 embryos when compared with ast^te284/te284 embryos. (I,R) Quantification of the number of hcrt:tdtomato longitudinal axons in outer or inner sections in ast^ti272z (I) or ast^te284 (R). Brackets in B,C,F,K,O indicate outer and inner sections used for quantification, defined by Mauthner neuron somata (outer section) and the area in between (inner section). Asterisks and plus signs indicate longitudinal hcrt:tdtomato axons in outer and inner sections, respectively. Scale bars: in A, 50 μm for A,E,J,N; in B, 25 μm for B-D,F-H,K-M,O-Q.