FIGURE

Fig. 5

ID

ZDB-FIG-121109-1

Publication

Kizil et al., 2012 - The chemokine receptor cxcr5 regulates the regenerative neurogenesis response in the adult zebrafish brain

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Fig. 5

Knocking down cxcr5 with cerebroventricular microinjection (CVMI) results in reduced regenerative neurogenesis but not radial glial cell proliferation. (A) Lesion, CVMI and BrdU treatment scheme. (B) BrdU and S100β immunohistochemistry (IHC) with 4,6-diamidino-2-phenylindole (DAPI) counterstaining on control morpholino-injected brains. (B′) BrdU channel. (B′′) S100β channel. (C) BrdU and S100β IHC with DAPI counterstaining on cxcr5 antisense morpholino-injected brains. (C’) BrdU channel. (C′′) S100β channel. (D) Graph showing the average number of proliferating glial cells. Knocking down cxcr5 does not alter the levels of proliferating radial glial cells. (E) Lesion, CVMI, BrdU treatment and pulse-chase scheme. (F) BrdU and HuC IHC with DAPI counterstaining on control morpholino-injected brains. (F′) BrdU channel. (F′′) HuC channel. (G) BrdU and HuC IHC with DAPI counterstaining on cxcr5 antisense morpholino-injected brains. (G′) BrdU channel. (G′′) HuC channel. (H) Graph showing the average number of newborn neurons. Knocking down cxcr5 significantly reduces the levels of regenerative neurogenesis.

Expression Data

Gene:	elavl3
Antibodies:	Ab1-elavl Ab1-s100
Fish:	WT WT + MO1-cxcr5
Condition:	physical alteration: anatomical structure
Knockdown Reagent:	MO1-cxcr5
Anatomical Terms:	telencephalon neuron telencephalon radial glial cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-cxcr5
Condition:	physical alteration: anatomical structure
Knockdown Reagent:	MO1-cxcr5
Observed In:	neurogenesis tissue regeneration
Stage:	Adult

Phenotype Detail

Acknowledgments

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