FIGURE

Fig. 5

ID

ZDB-FIG-120525-6

Publication

Mattes et al., 2012 - Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain

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Fig. 5

Primordia of the prethalamus and the thalamus are not affected in Wnt3/Wnt3a morphant embryos. Analysis of the fezf2 positive anterior forebrain area at 15 and 28 hpf reveals an expansion of the telencephalic domain (red brackets), however, the prethalamus (yellow brackets) is unaltered wnt3/wnt3a morphant embryos (A, B, G, H). Posteriorly analysis of the irx1b as well as the otx1l expression shows that the thalamic domain does not change significantly (yellow brackets), whereas, the midbrain territory shrinks in compound morphant embryos (red brackets, C-F; I-L). Notably, although both organizers - the MDO and MHB - disappeared in wnt3/wnt3a morphant embryos, expression of competence factors like fezf2 and irx1b are unaltered at 28 hpf, suggesting a specific function of Wnt3/Wnt3a in organizer formation. cFb, caudal forebrain, Mb, midbrain.

Expression Data

Genes:	fezf2 irx1b otx2a shha
Fish:	WT WT + MO1-wnt3 + MO2-wnt3a
Knockdown Reagents:	MO1-wnt3 MO2-wnt3a
Anatomical Terms:	dorsal thalamus forebrain mid diencephalic organizer central region mid diencephalic organizer dorsal region mid diencephalic organizer ventral region midbrain telencephalon ventral thalamus
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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