FIGURE

Fig. 4

ID

ZDB-FIG-120320-7

Publication

Takesono et al., 2012 - Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo

Other Figures

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Fig. 4

Slc3a2 inhibits the RhoA/ROCK pathway via c-Src activation in regulating YSL organization. (A) slc3a2 knockdown reduces tyrosine phosphorylation of c-Src. Western blot shows pY416 c-Src and total c-Src in lysates from control, slc3a2-morphants (MOs), or slc3a2/RhoA morphants (MOs+RhoA-MO). Data shown are representative of three independent experiments. (B) Overexpression of v-Src restores YSN localization after slc3a2 knockdown. The YSN are visualized by Sytox green. Clustered YSN in MOs (white arrows) are restored by coinjecting v-Src mRNA (v-Src+MOs). (C) Overexpression of v-Src restores microtubule networks in the YSL in slc3a2-knockdown embryos. YSL microtubule networks (αTUB; red), YSN distribution (SytG; green), and overlap of YSN and YSL microtubules (white squares in merged images) are all restored by v-Src+MOs.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-slc3a2a + MO1-slc3a2b + MO2-slc3a2a + MO2-slc3a2b
Knockdown Reagents:	MO1-slc3a2a MO1-slc3a2b MO2-slc3a2a MO2-slc3a2b
Observed In:	regulation of Rho-dependent protein serine/threonine kinase activity
Stage:	Dome

Phenotype Detail

Acknowledgments

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