ZFIN Image: Takesono et al., 2012, Fig. 4

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Fig. 4

Slc3a2 inhibits the RhoA/ROCK pathway via c-Src activation in regulating YSL organization. (A) slc3a2 knockdown reduces tyrosine phosphorylation of c-Src. Western blot shows pY416 c-Src and total c-Src in lysates from control, slc3a2-morphants (MOs), or slc3a2/RhoA morphants (MOs+RhoA-MO). Data shown are representative of three independent experiments. (B) Overexpression of v-Src restores YSN localization after slc3a2 knockdown. The YSN are visualized by Sytox green. Clustered YSN in MOs (white arrows) are restored by coinjecting v-Src mRNA (v-Src+MOs). (C) Overexpression of v-Src restores microtubule networks in the YSL in slc3a2-knockdown embryos. YSL microtubule networks (αTUB; red), YSN distribution (SytG; green), and overlap of YSN and YSL microtubules (white squares in merged images) are all restored by v-Src+MOs.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA