FIGURE

Fig. 2

ID

ZDB-FIG-120320-5

Publication

Takesono et al., 2012 - Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo

Other Figures

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Fig. 2

Loss of function of Slc3a2 enhances cell–cell fusion and YSN clustering. (A) Sequences of TPF time-lapse images showing GFP membrane (Upper) and outlines of marginal cells and the YSL (Lower). Fusing cells are indicated by colored outlines, and fusion locations are indicated by colored arrows. The width of the YSL at the outer edge is shown by a white bracket. Cell–cell fusion occurs mostly around the 512-cell stage (red arrow in control, red and green arrows in MOs) but persists through the following stages in slc3a2-MOs (MOs) (pink and cyan arrows in MOs). (B) Merged images of CARS (for lipid; blue) and TPF (for GFP membrane; green) of control and MOs. Uniformly distributed YSN in the control are indicated by white arrowheads, and clustered YSN in MOs are indicated by red asterisks. Multinucleated EVL cells are indicated by red brackets.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-slc3a2a + MO1-slc3a2b + MO2-slc3a2a + MO2-slc3a2b
Knockdown Reagents:	MO1-slc3a2a MO1-slc3a2b MO2-slc3a2a MO2-slc3a2b
Observed In:	external yolk syncytial layer syncytium formation by plasma membrane fusion yolk syncytial layer nucleus
Stage Range:	1k-cell to Oblong

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA