FIGURE

Fig. 2

ID

ZDB-FIG-120316-103

Publication

Kirchmaier et al., 2012 - The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Aberrant skeletal muscle development in popdc2 morphants. Skeletal muscle tissue formation in the trunk of (A–C) 24 hpf and (D–F) 72 hpf embryos, which were (A,D) wild type (WT), or injected with (B,E) MO1-popdc2, and (C,F) MO1/MO2-popdc2 morpholinos, respectively. Skeletal muscle development was studied by confocal microscopy using rhodamine-phalloidin staining. (A′–F′) depict the morphology of single muscle segments at higher magnification, which are labeled in (A–F) with a white square. The trunk musculature is highly aberrant in the morphants. Segment borders are not well developed and variable in size (square brackets in B). Moreover, muscle fibers are missing in some areas (arrows in E, F) or are detached (arrowheads in C). Scale bar = 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-popdc2 WT + MO1-popdc2 + MO2-popdc2
Knockdown Reagents:	MO1-popdc2 MO2-popdc2
Observed In:	myotome myotome myofibril skeletal muscle cell
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 363(2), Kirchmaier, B.C., Poon, K.L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., Stainier, D.Y., and Brand, T., The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development, 438-450, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.