FIGURE

Fig. 3

ID

ZDB-FIG-120316-104

Publication

Kirchmaier et al., 2012 - The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development

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Fig. 3

Fast and slow muscle formation in popdc2 morphants. Confocal imaging of transverse sections (A,D,G,J) through the 27 hpf trunk and lateral flatmounts of (B,E,H,K) 1 dpf and (C,F,I,L) 3 dpf embryos, which were injected with (A–C,G–I) MO-control (MO-CTR) or (D–F,J–L) MO1/MO2-popdc2 morpholinos, respectively and stained (A–F) with F310 antibody to visualize fast muscle fibers, and (G–L) with F59 antibody to detect slow muscle fibers. Sections stained for slow muscle fibers (G,J) were also stained with phalloidin detecting F-actin to visualize the fast muscle compartment (green). Asterisks in (K,L) pointing to gaps in the fiber arrangement. Arrows in (F) indicate ruptured fast muscle fibers and in (L) point to slow muscle fibers with a wavy morphology. Scale bars = 50 μm.

Expression Data

Antibodies:	Ab-F59 Ab-F310
Fish:	WT WT + MO1-popdc2 + MO2-popdc2
Knockdown Reagents:	MO1-popdc2 MO2-popdc2
Anatomical Terms:	fast muscle cell slow muscle cell
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-popdc2 + MO2-popdc2
Knockdown Reagents:	MO1-popdc2 MO2-popdc2
Observed In:	fast muscle cell skeletal muscle cell slow muscle cell
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 363(2), Kirchmaier, B.C., Poon, K.L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., Stainier, D.Y., and Brand, T., The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development, 438-450, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.