ZFIN Image: Kirchmaier et al., 2012, Fig. 2

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Figure Caption

Fig. 2 Aberrant skeletal muscle development in popdc2 morphants. Skeletal muscle tissue formation in the trunk of (A–C) 24 hpf and (D–F) 72 hpf embryos, which were (A,D) wild type (WT), or injected with (B,E) MO1-popdc2, and (C,F) MO1/MO2-popdc2 morpholinos, respectively. Skeletal muscle development was studied by confocal microscopy using rhodamine-phalloidin staining. (A′–F′) depict the morphology of single muscle segments at higher magnification, which are labeled in (A–F) with a white square. The trunk musculature is highly aberrant in the morphants. Segment borders are not well developed and variable in size (square brackets in B). Moreover, muscle fibers are missing in some areas (arrows in E, F) or are detached (arrowheads in C). Scale bar = 100 μm.

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Acknowledgments

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Reprinted from Developmental Biology, 363(2), Kirchmaier, B.C., Poon, K.L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., Stainier, D.Y., and Brand, T., The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development, 438-450, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.