FIGURE

Fig. 8

ID

ZDB-FIG-111117-20

Publication

Xi et al., 2011 - Transgenic zebrafish expressing green fluorescent protein in dopaminergic neurons of the ventral diencephalon

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Fig. 8

Effects of MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) on green fluorescent protein (GFP) -positive neurons in the ventral diencephalon. Tg(dat:EGFP) embryos were untreated (Ctrl) or treated with MPTP at 1 mM from 24 hours post-fertilization (hpf), and examined under fluorescence microscope at 3 days post-fertilization (dpf) (A,B) and 5 dpf (C,D). The numbers of GFP-positive neurons in the ventral diencephalon (dopaminergic [DA] neuron groups 2–6) were counted and statistically analyzed (E; **P < 0.01, ***P < 0.001). F–G23: Double immunostaining for GFP and tyrosine hydroxylase (TH) on Ctrl or MPTP-treated embryos at 3 dpf. A–D: Anterior is to the bottom; F–G23: Anterior is to the left. More than 30 larvae were examined in each group. Scale bars = 25 μm.

Expression Data

Genes:	EGFP th
Antibody:	Ab4-th
Fish:	ot80Tg
Condition:	chemical treatment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Anatomical Terms:	diencephalon ventral region dopaminergic neuron
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ot80Tg
Condition:	chemical treatment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Observed In:	diencephalon ventral region dopaminergic neuron
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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