|
Fig. 8
Effects of MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) on green fluorescent protein (GFP) -positive neurons in the ventral diencephalon. Tg(dat:EGFP) embryos were untreated (Ctrl) or treated with MPTP at 1 mM from 24 hours post-fertilization (hpf), and examined under fluorescence microscope at 3 days post-fertilization (dpf) (A,B) and 5 dpf (C,D). The numbers of GFP-positive neurons in the ventral diencephalon (dopaminergic [DA] neuron groups 2–6) were counted and statistically analyzed (E; **P < 0.01, ***P < 0.001). F–G23: Double immunostaining for GFP and tyrosine hydroxylase (TH) on Ctrl or MPTP-treated embryos at 3 dpf. A–D: Anterior is to the bottom; F–G23: Anterior is to the left. More than 30 larvae were examined in each group. Scale bars = 25 μm.