Fig. 2

Neuromast position, primordium kinetics and chemokine receptor expression are unaffected by loss of Lef1 function. (A-C) Histograms depicting the number/position of neuromasts in siblings (A) lef1^u767 mutants (B) and lef1 morphants (C) at 3 dpf. Arrows indicate the average position and colours denote the different populations of sequentially deposited neuromasts. (D,E) Plot of the distance (in μm) in lef1^u767 mutants (D) and lef1 morphants (E) between the caudal limit of the otic vesicle and the leading edge of the PLLP at 27, 30, 33 and 36 hpf (n=7 mutants/morphants and n=15 wild types in both cases). The only significant difference is observed at 36 hpf (^*P<0.01, Wilcoxon rank-sum test). Data are mean+s.d. (F,G) GFP [Tg(-8.0cldnb:lynEGFP)^zf106]-positive cells extend beyond the terminal neuromast in 55 hpf lef1^u767 embryos (arrowhead). Arrows indicate neuromasts L4 and L5. Nuclei are counterstained with propidium iodide. Scale bar: 20 μm. (H-O) Expression of cxcr4b (H-K) and cxcr7b (L-O) in wild type (H,I,L,M) and lef1^u767 mutants (J,K,N,O) at 33 and 36 hpf.