Fig. S4
Relationship between the hindbrain CtAs and zn-8 positive neurons and axons at 72 hpf. A-D, Maximum intensity confocal projections of immuno-fluorescently stained embryos carrying the endothelial reporter Tg(kdrl:GFP)1a116. Endothelium, green (GFP). zn-8 positive (DM-GRASP/Neurolin) neurons and axons, red (A,B) or white (C,D). A,C, dorsal views. Anterior, left. Left side, bottom. B,D, left lateral views. Anterior, left. Dorsal, top. Abbreviations (see Table 1): vasculature, white; neurons and commissures, yellow. Small white arrows, CtAs. Small yellow arrowheads, prominent clusters of neurons in the neuroectoderm′s dorso-lateral region. Yellow brackets, ventral axonal commissures. Yellow asterisk, r5 GFP-positive neuroepithelial signal from the Tg(kdrl:GFP)1a116 reporter. The abducens (VI) motor neurons, the abducens nerves (VIn) and the cerebellar plate (CP) are labeled. Scale bar (A), 100 μm. |
Reprinted from Developmental Biology, 357(1), Ulrich, F., Ma, L.H., Baker, R.G., and Torres-Vazquez, J., Neurovascular development in the embryonic zebrafish hindbrain, 134-51, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.