Fig. S4
Relationship between the hindbrain CtAs and zn-8 positive neurons and axons at 72 hpf. A-D, Maximum intensity confocal projections of immuno-fluorescently stained embryos carrying the endothelial reporter Tg(kdrl:GFP)1a116. Endothelium, green (GFP). zn-8 positive (DM-GRASP/Neurolin) neurons and axons, red (A,B) or white (C,D). A,C, dorsal views. Anterior, left. Left side, bottom. B,D, left lateral views. Anterior, left. Dorsal, top. Abbreviations (see Table 1): vasculature, white; neurons and commissures, yellow. Small white arrows, CtAs. Small yellow arrowheads, prominent clusters of neurons in the neuroectoderm′s dorso-lateral region. Yellow brackets, ventral axonal commissures. Yellow asterisk, r5 GFP-positive neuroepithelial signal from the Tg(kdrl:GFP)1a116 reporter. The abducens (VI) motor neurons, the abducens nerves (VIn) and the cerebellar plate (CP) are labeled. Scale bar (A), 100 μm.
Reprinted from Developmental Biology, 357(1), Ulrich, F., Ma, L.H., Baker, R.G., and Torres-Vazquez, J., Neurovascular development in the embryonic zebrafish hindbrain, 134-51, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.