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Putiri et al., 2011 - The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes

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Fig. s6

Maternal mis function is not required for the activation of target genes by ectopic Wnt/Β-catenin and FGF signaling. Embryos were assayed for goosecoid expression at 70% epiboly (8 h.p.f.; A–F) or chordin expression at 30% epiboly (5 h.p.f.; G–J). Uninjected wild-type (A,G) and mis (B,H) show dorsally restricted expression of gsc and chd. Wild-type embryos injected with ß-catenin (C,I) or fgf8a (E) mRNA show expanded domains of dorsal gene expression. mis mutant embryos injected with ß-catenin (D,J) or fgf8a (F) mRNA also show ectopic expression of dorsal genes, although, as noted in Supp. Fig. 4, the overall levels of gsc expression can be reduced.

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Acknowledgments

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Reprinted from Developmental Biology, 353(2), Putiri, E., and Pelegri, F., The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes, 275-289, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.