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Fig. 6

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ZDB-FIG-110624-44

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Putiri et al., 2011 - The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes

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Fig. 6

Nodal-dependent induction of endoderm-specific genes in blastoderm explants is dependent on maternal mis function. (A) Experimental manipulation: cyc mRNA was injected at the 1-cell stage, and the blastoderm was manually excised away from marginal cells and the yolk cell at the late blastula stage (sphere stage, 4 h.p.f.), as originally carried out by David and Rosa (2001). At 7 h.p.f., explants from wild-type embryos show robust sox17 expression (B), while explants from mis mutant embryos lack sox17 expression (C). Embryos shown are a subset of representative embryos from two experiments with at least 15 wild-type and 15 mis embryos per experiment. Embryonic explants were only included if they were intact after dissection. Embryo diagrams in (A) are adapted from Kimmel et al. (1995) (images courtesy of ZFIN).

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Reprinted from Developmental Biology, 353(2), Putiri, E., and Pelegri, F., The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes, 275-289, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.