FIGURE

Fig. 4

ID

ZDB-FIG-110214-37

Publication

Ravanelli et al., 2011 - The Actin Nucleator Cordon-bleu is Required for Development of Motile Cilia in Zebrafish

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Fig. 4

cobl morphants display abnormal left–right molecular asymmetry.

RNA in situ hybridization of spaw (A-D) in control and morphant embryos from 16 to 20 somites. (E) Bar graph showing the percentage of treated embryos displaying spaw expression on the left, right (R), bilaterally (Bi) or absent expression (Abs). RNA in situ hybridization of pitx2 (F–I) in control and morphant embryos from 19 to 23 somites. (J) Bar graph showing the percentage of treated embryos displaying the pitx2 expression pattern seen in F–I. Normally, spaw and pitx2 expression is induced only in the left lateral plate mesoderm by signals originating from Kupffer′s vesicle. Morphants express these genes on either the left or right sides, both sides, or not at all. Arrows indicate expression domain of spaw and pitx2.

Expression Data

Genes:	pitx2 spaw
Fish:	EKW EKW + MO1-cobl
Knockdown Reagent:	MO1-cobl
Anatomical Terms:	lateral plate mesoderm left side lateral plate mesoderm right side
Stage Range:	14-19 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	EKW + MO1-cobl
Knockdown Reagent:	MO1-cobl
Observed In:	determination of left/right asymmetry in lateral mesoderm
Stage Range:	14-19 somites to 20-25 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 350(1), Ravanelli, A.M., and Klingensmith, J., The Actin Nucleator Cordon-bleu is Required for Development of Motile Cilia in Zebrafish, 101-111, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.