FIGURE

Fig. 7

ID

ZDB-FIG-110214-19

Publication

Tittle et al., 2011 - Uhrf1 and Dnmt1 are required for development and maintenance of the zebrafish lens

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Lens fibers express markers of terminal differentiation in uhrf1 and dnmt1 mutants. (A–I) Cryosections from 5 dpf embryos immunostained for the lens fiber markers Crystallin AlphaA (A–C, red), Lengsin (D–F, red), or Aquaporin 0 (G–I, red). In all sections, nuclei are counterstained with Sytox-Green (green). uhrf1 (B,E,H) and dnmt1 (C,F,I) fibers express these markers, indicating that the disorganized secondary fibers in the mutant lenses still express markers of terminal differentiation. Yellow arrows in panels C, E, and F indicate regions of the mutant cornea which ectopically express lens fiber genes. In all panels, mild mutants were selected for analysis. Anterior is to the right in all panels. Scale bars are 80 μm.

Expression Data

Gene:	lgsn
Antibody:	Ab1-lgsn
Fish:	WT dnmt1^s872/s872 uhrf1^{hi3020Tg/hi3020Tg}
Anatomical Term:	lens
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 350(1), Tittle, R.K., Sze, R., Ng, A., Nuckels, R.J., Swartz, M.E., Anderson, R.M., Bosch, J., Stainier, D.Y., Eberhart, J.K., and Gross, J.M., Uhrf1 and Dnmt1 are required for development and maintenance of the zebrafish lens, 50-63, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.