FIGURE

Fig. 7

ID

ZDB-FIG-100806-79

Publication

Francescatto et al., 2010 - The activation of membrane targeted CaMK-II in the zebrafish Kupffer's vesicle is required for left-right asymmetry

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Fig. 7

CaMK-II activation is disrupted in morphants. Confocal immunofluorescent projections of P-T²⁸⁷ CaMK-II (red) and cilia (green) in embryos at the 12-somite stage after injection with (A) 5 ng mismatch (control), (B) 1.5 ng camk2aKAP, (C) 1.5 ng camk2b2, (D) 1.25 ng camk2g1 MO, (E) 150 ng Sox17-K⁴³A GFP-CaMK-II cDNA (inset is green fluorescence of cilia and GFP-CaMK-II around the KV), (F) 4 ng ntl MO, (G) 4 ng pkd2 MO or (H) 4 ng ryr3 MO. Scale bar: 10 μm.

Expression Data

Antibodies:	Ab1-camk2 Ab1-tuba
Fish:	WT WT + MO1-camk2g1 WT + MO1-ryr3 WT + MO1-tbxta WT + MO2-camk2a WT + MO2-camk2b2 WT + MO3-pkd2
Knockdown Reagents:	MO1-camk2g1 MO1-ryr3 MO1-tbxta MO2-camk2a MO2-camk2b2 MO3-pkd2
Anatomical Terms:	Kupffer's vesicle ciliated cell Kupffer's vesicle cilium
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-camk2g1 WT + MO1-ryr3 WT + MO1-tbxta WT + MO2-camk2a WT + MO2-camk2b2 WT + MO3-pkd2
Knockdown Reagents:	MO1-camk2g1 MO1-ryr3 MO1-tbxta MO2-camk2a MO2-camk2b2 MO3-pkd2
Observed In:	calcium/calmodulin-dependent protein kinase activity Kupffer's vesicle calcium- and calmodulin-dependent protein kinase complex protein autophosphorylation
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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