FIGURE

Fig. 3

ID

ZDB-FIG-100630-1

Publication

Nakano et al., 2010 - Biogenesis of GPI-anchored proteins is essential for surface expression of sodium channels in zebrafish Rohon-Beard neurons to respond to mechanosensory stimulation

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Fig. 3

Mutant RB neurons fail to generate normal action potentials owing to defective sodium currents. (A) Injection of step currents up to 100 pA elicited typically large action potentials in a wild-type RB neuron. (B) Current injection into a mutant RB cell failed to generate normal action potentials. (C) The spikes in wild-type RB neurons were eliminated by bath application of TTX, a sodium channel blocker. (D) The small, partial spikes in mutant RB neurons were also eliminated by TTX. (E) Inward sodium currents elicited by voltage steps from -100 mV to 0 mV were recorded in wild-type RB neurons. (F) Inward sodium currents in mutant RB neurons were observed but with smaller amplitude than those seen in wild-type neurons. (G) The inward sodium currents in wild type were blocked in the presence of TTX. (H) The sodium currents in mutant RB cells were also eliminated by TTX.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pigu^mi310/mi310
Observed In:	neuronal action potential Rohon-Beard neuron voltage-gated sodium channel activity
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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