FIGURE

Fig. 6

ID

ZDB-FIG-100611-6

Publication

Riley et al., 2004 - Rhombomere boundaries are Wnt signaling centers that regulate metameric patterning in the zebrafish hindbrain

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Fig. 6

Hindbrain patterning in Delta-Notch mutants. A-C: Hindbrain morphology at 24 hours postfertilization (hpf) in wild-type (A), dlA^dx2 (B), and mib^ta52b (C) embryos. Positions of rhombomeres 1-7 are indicated. D-F: Dorsal views, showing expression of foxb1.2 at 24 hpf in wild-type (D), dlA^dx2 (E), and mib^ta52b (F) embryos. G: erm expression in mib^ta52b at 30 hpf. The otic vesicles (ov) are indicated to serve as landmarks. H,I: ephA4 expression at 24 hpf in a wild-type embryo (H) and a mib^ta52b mutant (I). J,K: Expression of krox20 at 24 hpf in dlA^dx2 (J) and mib^ta52b (K) embryos. L,M: Expression of hoxb1a at 24 hpf in dlA^dx2 (L) and mib^ta52b (M) embryos. N: Reticulospinal neurons (rsn) in a mib^ta52b mutant labeled with anti-acetylated tubulin at 24 hpf. O: zn8 antibody staining of a mib^ta52b mutant at 30 hpf shows that commissural neurons (cn) are not produced. P,Q: Mauthner neurons (Mn) labeled with 3A10 antibody at 30 hpf in dlA^dx2 (P) and mib^ta52b (Q) embryos. Abnormal axonal trajectories are indicated (arrows). R: Radial glia (rg) labeled with zrf1-4 antibodies at 30 hpf in a mib^ta52b mutant. Images show lateral views with anterior to the left (A-C,H,I,R) or dorsal views with anterior to the top (D-G,J-Q). Scale bar in R = 55 μm in A-C, 75 μm in O-Q, 100 μm in R,125 μm in D-N.

Expression Data

Genes:	dla egr2b epha4a etv5b foxb1a hoxb1a
Antibodies:	Ab-3A10 Ab1-tuba zn-8 zrf-1 zrf-2 zrf-3 zrf-4
Fish:	AB dla^dx2/dx2 mib1^ta52b/ta52b
Anatomical Terms:	hindbrain hindbrain primary neuron Mauthner neuron otic vesicle radial glial cell rhombomere rhombomere 3 rhombomere 4 rhombomere 5
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dla^dx2/dx2 mib1^ta52b/ta52b
Observed In:	axon guidance embryonic pattern specification hindbrain hindbrain radial glial cell hindbrain commissure neuron Mauthner neuron Mauthner neuron axon primary neuron axon rhombomere rhombomere primary neuron
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

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