FIGURE

Fig. S6

ID

ZDB-FIG-091221-27

Publication

Song et al., 2009 - Mechanisms Underlying Metabolic and Neural Defects in Zebrafish and Human Multiple Acyl-CoA Dehydrogenase Deficiency (MADD)

Other Figures

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Fig. S6

xav mutants exhibit reduced motor axon branching and neuromuscular synaptogenesis that are not caused by change of motor neuron number or viability. A. SV2 (green) and AChR (red) labeling showed that motor axon branching and neuromuscular synaptogenesis were reduced in xav mutants and etfdh morphants compared to WT embryos, at 56 hpf and most strikingly at 72 hpf. Scale bar = 20 μm. B. Motor neuron number, as assayed by in situ hybridization for islet-2, is similar between xav mutants and WT embryos at 48 hpf. Scale bar = 100 μm. C. No substantial apoptosis was seen in the pool of motor neurons in xav compared to WT at 48 hpf, as assayed by double staining for TUNEL and Zn5, which labels secondary motor neurons. N>10 embryos, 3 carrier pairs. Scale bar = 20 μm.

Expression Data

Genes:	alcama chrna1 isl2a
Antibodies:	Ab-SV2 zn-5
Fish:	WT etfdh^p6di/p6di WT + MO1-etfdh
Knockdown Reagent:	MO1-etfdh
Anatomical Terms:	motor neuron axon muscle cell acetylcholine-gated channel complex myotome neuromuscular junction Rohon-Beard neuron secondary motor neuron axon secondary motor neuron cell body spinal cord motor neuron
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	etfdh^p6di/p6di
Observed In:	motor neuron motor neuron axon motor neuron axon terminus myotome neuromuscular junction secondary motor neuron
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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