Fig. 4

Effect of inhibiting FoxD5 protein synthesis on the expression of mespa and mespb. By WISH, the expressions of mespa, mespb and papc in wild-type embryos (WT) and FoxD5 morphants (FoxD5 MO) were observed at 12–24 hpf as indicated. mespa was expressed at somites S-I and S-II in WT (A), but was greatly reduced in FoxD5 morphants (B). mespb was detected from S0 to S-II as a striped pattern in WT (C), but failed to maintain a striped expression (D). Expression of tbx24 was detected in the PSM and the anterior part of formed somites from S0 to SII in WT (arrowheads in E). Although tbx24 expression was unchanged in the PSM, it failed to maintain a segmented expression at the somite region in the FoxD5 morphants (arrows in F). FoxD5 was expressed at tailbud, adaxial cells, and forming somites in WT (arrowheads in G), whereas FoxD5 was still expressed at tailbud and adaxial cells, but was not at the forming somite region in the tbx24 morphants (H).