Fig. 6

Hh activity was required for inhibition of FoxD5 expression in the somites. WISH was used to detect the expression of FoxD5, myod, mespa, mespb and ripply1 in control (EtOH) embryos and cyclopamine-treated embryos, as indicated. Embryos were incubated for 6 h in ethanol (A–E) or cyclopamine (F–J), starting at 6 hpf, followed by WISH analysis at 12 hpf. The expression of FoxD5 in the adaxial cells was greatly reduced when Hh activity was inhibited (B). Interestingly, a slightly enhanced and extended expression of FoxD5 in the anterior PSM and somites was observed in cyclopamine-treated embryos (B). In WT embryos, myod was detected in the somites and adaxial cells (C). However, although myod could still be detected in the somites, it was completely lost in the adaxial cells of cyclopamine-treated embryos (D). Both mespa and mespb were normally expressed in control embryos (C, D) and cyclopamine-treated embryos (H, I). The repressor ripply1 was normally detected in control embryos (E) and cyclopamine-treated embryos (J).