Loss of FoxD5 function did not affect the wavefront and clock in the PSM, but did cause defects in the somites and anterior PSM where the somites were formed. By WISH, the expressions of deltaC, her1, notch2, notch3, ephB2a, paraxis and tbx24, as well as FoxD5 in wild-type embryos (WT), FoxD5 morphants (FoxD5 MO) and tbx24 morphants (tbx24 MO), were observed at 10–14 hpf as indicated. Compared to WT embryos (A, C), FoxD5 morphants showed normal variation in the pattern of deltaC expression, but the striped expression was not maintained in the region where the somites (arrowhead) should have been presented (B, D). The expression of her1 showed a normal oscillation in WT (E) and FoxD5 morphants (F). The striped patterns of notch2, notch3, ephB2a and paraxis in the somites were normal in WT embryos (G, I, K, M), but they were disordered in FoxD5 morphants (H, J, L, N). Expression of papc appeared strongly at somites from S-II to S0. Although the intensity was decreased from posterior to anterior in WT embryos (O), the expression of papc was ectopically increased and showed 5–6 strong signal bands in FoxD5 morphants (P).
|
