FIGURE

Fig. 4

ID

ZDB-FIG-090710-44

Publication

Lightcap et al., 2009 - Interaction with LC8 is required for Pak1 nuclear import and is indispensable for zebrafish development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Pak1 nuclear import is critical for zebrafish development and survival.

(A) Representative images of zebrafish embryos at 4 dpf. Embyros were co-injected with the morpholino (MO1) and either human wt-Pak1 mRNA, human Pak1-NLSmut or human Pak1-LC8mut (last three panels). Uninjected embryos and MO1 only injected embryos are also shown. (B–D) Quantitative analysis of differential zebrafish survival, body length, and extent of cardiac edema at 4 dpf in embryos injected with different morpholino/Pak1 mRNA combinations. For B, percent survival of uninjected control fish is approximately 90%. For D, cardiac edema for experimental fish was normalized to the control fish.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-pak1
Knockdown Reagent:	MO1-pak1
Observed In:	eye head heart post-vent region whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One