FIGURE

Fig. 4

ID

ZDB-FIG-081107-36

Publication

Davuluri et al., 2008 - Mutation of the zebrafish nucleoporin elys sensitizes tissue progenitors to replication stress

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Fig. 4

Nuclear pore disruption in flo mutants.

(A–C) Confocal projections through the posterior intestine of 75 hpf wild type (A), flo (B), and elys morpholino injected larvae (C), following anti-FG nucleoporin immunostainings with mAb414 (green; DAPI–blue). There is a dramatic reduction of nuclear pores in the flo and morpholino injected larvae. Inset shows higher magnification of localized regions of the DAPI-stained image. (D–F) Identical findings are evident in the retina of these larvae. Note apparent cytoplasmic accumulation of the immunoreactive FG-nucleoporins in flo and morpholino injected larvae. (G,H) Normal nuclear distribution of FG nucleoporins in wild type and flo skeletal muscle. (I) Western blot showing levels of FG nucleoporin proteins relative to beta-actin in nuclear (nucl) and cytoplasmic (cyto) extracts derived from the intestine of 75 hpf flo and sibling wild type larvae.

Expression Data

Antibody:	Ab1-nucleoporin
Fish:	WT ahctf1^{ti262c/ti262c} WT + MO1-ahctf1 + MO2-ahctf1
Knockdown Reagents:	MO1-ahctf1 MO2-ahctf1
Anatomical Terms:	intestine cytoplasm intestine nucleus posterior intestine nuclear pore retina nuclear pore skeletal muscle cell nuclear pore
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ahctf1^{ti262c/ti262c} WT + MO1-ahctf1 + MO2-ahctf1
Knockdown Reagents:	MO1-ahctf1 MO2-ahctf1
Observed In:	intestine nuclear pore posterior intestine nuclear pore retina nuclear pore skeletal muscle cell nuclear pore
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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