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Edg1 acts antagonistically against Mil in the directed migration of prechordal plate progenitor cells. (A-F) In situ hybridisation of dlx, hgg1 and ntl of WT embryos injected with 6.2 ng edg1-MO alone (A,D) or co-injected with 4.3 ng mil-MO (B,E) at tailbud stage, and of slb embryos injected with 6.2 ng edg1-MO (C,F). (G-I) In situ hybridisation of dlx3 and hlx1 of WT embryos injected with edg1-MO alone (G) or co-injected with mil-MO (H) at tailbud stage, and of slb embryos injected with edg1-MO (I). (A-C,G-I) Dorso-animal views; (D-F) lateral views. edg1-MO injection reduced anterior migration of the anterior (A,D) and posterior (G) prechordal plate, compared with WT (see Fig. 1A,E,I), and enhanced the slb prechordal plate phenotype (C,F,I) (see Fig. 1C,G,K). Co-injection of edg1-MO and mil-MO cancelled out the effect of one another and often gave rise to an embryo reminiscent to WT (B,E,H).
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