FIGURE

Fig. 5

ID

ZDB-FIG-080904-15

Publication

Sperber et al., 2008 - barx1 is necessary for ectomesenchyme proliferation and osteochondroprogenitor condensation in the zebrafish pharyngeal arches

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Fig. 5

Barx1 is necessary for expression of chondrogenic markers within the pharyngeal arches. (A, C, E, G, I and K) Control embryos; (B, D, F, H, J and L) barx1 morphant embryos (see Fig. 4). Whole-mount in situ hybridization for (A, B) goosecoid (gsc), (C, D) sox9a, (E, F) runx2b, (G, H) type II collagen (col2a1), (I, J) chondromodulin (chm), and (K, L) dlx2b. Anterior is to the left; open arrowheads indicate sites of altered marker expression in the morphants; bcc, basicranial commissures; cb, ceratobranchials; ch, ceratohyal; cl, cleithrum; d, diencephalon; ep, ethmoid plate; fb, fin bud; hsc, hyosymplectic condensation; ov, otic vesicle; pq, palatoquadrate; t, telencephalon. Scale bar: 100 μm.

Expression Data

Genes:	cnmd col2a1a dlx2b gsc runx2b sox9a
Fish:	EKW EKW + MO1-barx1 + MO2-barx1
Knockdown Reagents:	MO1-barx1 MO2-barx1
Anatomical Terms:	ceratobranchial 5 tooth ceratobranchial cartilage ceratohyal cartilage cleithrum cranial cartilage ethmoid cartilage hyosymplectic cartilage occipital region palatoquadrate cartilage pectoral fin
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	EKW + MO1-barx1 + MO2-barx1
Knockdown Reagents:	MO1-barx1 MO2-barx1
Observed In:	cartilage development ceratobranchial 5 tooth head odontogenesis pharyngeal arch
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 321(1), Sperber, S.M., and Dawid, I.B., barx1 is necessary for ectomesenchyme proliferation and osteochondroprogenitor condensation in the zebrafish pharyngeal arches, 101-110, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.