FIGURE

Fig. 4

ID

ZDB-FIG-080904-14

Publication

Sperber et al., 2008 - barx1 is necessary for ectomesenchyme proliferation and osteochondroprogenitor condensation in the zebrafish pharyngeal arches

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Fig. 4

Initial patterning of the arches is maintained in barx1 morphants. (A, C, E and G) Control MO-injected embryos, (B, D, F and H) embryos co-injected with Bx MO + Sa MO, 2 ng each. Neural crest expression of inca (A, B) and dlx2a (C and D) at 24 hpf. (E, F) neurod expression at 24 hpf in neural ganglia precursors; (G and H) hand2 expression in ventral endoderm at 32 hpf. Neural crest streams marked in Roman numerals, gill arches indicated by numbers; e, eye; m, mandibular arch; h, hyoid arch; ov, otic vesicle; ad/av/f, anterodorsal/anteroventral lateral line/facial placode/ganglia; o, octavel/statoacoustic ganglia precursors; p, posterior lateral line placode/ganglion. Scale bar: 100 μm.

Expression Data

Genes:	dlx2a hand2 inka1a neurod1
Fish:	EKW EKW + MO1-barx1 + MO2-barx1
Knockdown Reagents:	MO1-barx1 MO2-barx1
Anatomical Terms:	dorsal anterior lateral line ganglion facial placode mandibular arch skeleton neural crest olfactory placode pharyngeal arch 2 pharyngeal arch 3-7 pharyngeal endoderm posterior lateral line ganglion statoacoustic (VIII) ganglion telencephalon ventral anterior lateral line ganglion ventral thalamus
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	EKW + MO1-barx1 + MO2-barx1
Knockdown Reagents:	MO1-barx1 MO2-barx1
Observed In:	embryo development
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 321(1), Sperber, S.M., and Dawid, I.B., barx1 is necessary for ectomesenchyme proliferation and osteochondroprogenitor condensation in the zebrafish pharyngeal arches, 101-110, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.