FIGURE

Fig. 3

ID

ZDB-FIG-080328-7

Publication

Liedtke et al., 2008 - Midkine-b regulates cell specification at the neural plate border in zebrafish

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Fig. 3

A: mdkb splice-site Morpholinos (MOs) block normal processing of endogenous mdkb pre-mRNA. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of mdkb transcription in uninjected embryos (lane 1), embryos injected with one MO (Up, lane 2; Down, lane 3) and embryos co-injected with both splice-site MOs (lane 4). Lane M represents a 100-bp DNA size ladder (Invitrogen). The schematic drawing shows the organization of the mdkb pre-mRNA (red and yellow dots indicate blocked splice sites), intron sizes and regions of primer binding. B-E: No effects of reduced Mdkb activity on myoD expression in the adaxial mesoderm were observed, while foxd3 expression is reduced in premigratory ncc. Dorsal views of anterior (B,D), respectively posterior trunk regions (C,E) of the same embryos are shown. F-I: Normal hindbrain patterning in mdkb morphants, as evident by regular egr2b expression in rhombomeres r3 and r5, and hoxb1a expression in r4. J-M: No effects of reduced Mdkb activity on foxa2 expression in the axial mesoderm and on dlx3b expression, indicating regular convergence and size of the neural plate.

Expression Data

Genes:	dlx3b egr2b foxa2 foxd3 hoxb1a myod1
Fish:	AB AB + MO1-mdkb + MO2-mdkb
Knockdown Reagents:	MO1-mdkb MO2-mdkb
Anatomical Terms:	adaxial cell axial mesoderm neural crest cell neural plate presumptive rhombomere 3 presumptive rhombomere 4 presumptive rhombomere 5
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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