FIGURE

Fig. 4

ID

ZDB-FIG-080326-88

Publication

Nagayoshi et al., 2008 - Insertional mutagenesis by the Tol2 transposon-mediated enhancer trap approach generated mutations in two developmental genes: tcf7 and synembryn-like

Other Figures

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Fig. 4

Roles of Tcf7 and Lef1 in the pectoral fin outgrowth. (A-C) Microinjection of lef1-MO into HG21C embryos. The pectoral fins of MO-injected heterozygous (A) and homozygous (B) embryos at 72 hpf. (C) Summary of the numbers of MO-injected embryos that showed an outgrown fin or reduced fin phenotype. (D-U) The pectoral fins of lef1-MO-injected wild-type and HG21C embryos analyzed by whole-mount in situ hybridization. The stages (28 hpf, 36 hpf, 38 hpf and 48 hpf) are shown on the top right. Wild-type (D-H,J,L,N,P,R), heterozygous (T) and homozygous (I,K,M,O,Q,S,U) embryos were used. The probes used are indicated (top right).

Expression Data

Genes:	dlx2a fgf8a fgf10a fgf24 lef1 tcf7 wnt3a
Fish:	WT WT + MO3-lef1 tcf7^{nkhg21cEt/nkhg21cEt} + MO3-lef1
Knockdown Reagent:	MO3-lef1
Anatomical Terms:	apical ectodermal ridge pectoral fin bud fin fold pectoral fin bud mesenchyme pectoral fin pectoral fin bud pectoral fin bud mesenchyme
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tcf7^nkhg21cEt/+ + MO3-lef1 tcf7^{nkhg21cEt/nkhg21cEt} + MO3-lef1
Knockdown Reagent:	MO3-lef1
Observed In:	pectoral fin
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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