nr4a2a is expressed in a population of differentiating neurons. (A-H″) Dorsal views of 48 hpf embryos hybridized with lmx1b.1 and sox2 (A-B″), nr4a2a and sox2 (C-D″), nr4a2a and gfap (E-F″), or nr4a2a and elavl3 (G-H″) probes show that nr4a2a is not expressed in progenitor cells, but in early differentiating cells. A, C, E and G show dorsal overviews of the head, at the level of the ventral diencephalon. High magnifications of the posterior tubercular area are reported on the right side of each overview. All the images are single confocal planes, except G, which is a 22 μm confocal projection. Anterior is to the left. Scale bars: 50 μm.
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