Fig. 5

Genetic Interactions between tob1a and Maternal or Zygotic Wnt/β-Catenin Signals (A) Interaction between tob1a and maternal Wnt/β-catenin activity. After injection with 20 ng βcat2MO, 20 ng tob1a-MO, or both (indicated on the top) at the one-cell stage, embryos were examined for boz and chordin (chd) expression at the 30% epiboly stage and for otx2 and hoxb1b expression at the 90% epiboly stage (indicated on the right). Embryos are shown in lateral views with dorsal oriented toward the right for boz and hoxb1b, in animal pole views with dorsal oriented toward right for chd, and in anterodorsal views with anterior oriented toward the top for otx2. (B) Effect of overexpression of tob1a mutants on early dorsoventral patterning. Embryos were injected with 400 pg of each mRNA species at the one-cell stage and were examined for boz expression at the oblong stage and for chd and goosecoid (gsc) expression at the shield stage. Embryos are shown in lateral views with dorsal oriented toward the right for boz, in animal pole views with dorsal oriented toward the right for chd, and in dorsal views with animal pole oriented toward the top for gsc. For details for tob1a mutants, see Figure 3F. (C) Interaction between tob1a and zygotic Wnt/β-catenin activity. Injections were done at the one-cell stage at the dose of 20 ng for tob1a-MO, 8 ng for wnt8MO2, or 400 pg for tob1a mRNA. Embryos are shown in dorsal views with dorsal oriented toward the right for chd at the shield stage, in lateral views with dorsal oriented toward the right for tbx6 at the 75% epiboly stage, and in anterodorsal views with anterior oriented toward the top for otx2 at the 90% epiboly stage.