FIGURE

Fig. 7

ID

ZDB-FIG-070802-53

Publication

Gansner et al., 2007 - Essential role of lysyl oxidases in notochord development

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Fig. 7

Expression of col2a1 mRNA by notochord vacuolar cells persists late following lysyl oxidase inhibition. Wild-type embryos were injected with 7.4 ng control morpholino (A), incubated in 10 μM neocuproine (B), incubated in 10 mM β-aminopropionitrile supplemented with 10 μM CuCl₂ (C), or injected with 3.7 ng each of loxl1 and loxl5b morpholino (D). In situ hybridization was carried out at 24 hpf, and frozen sections confirmed persistent col2a1 expression following lysyl oxidase inhibition (B–D, arrowhead), as well as floorplate and hypochord staining (A–D, arrows).

Expression Data

Gene:	col2a1a
Fish:	WT WT + MO1-loxl1 + MO1-loxl5b
Conditions:	chemical treatment: beta-aminopropionitrile hemifumarate chemical treatment: neocuproine
Knockdown Reagents:	MO1-loxl1 MO1-loxl5b
Anatomical Terms:	floor plate hypochord notochord
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-loxl1 + MO1-loxl5b WT + MO1-loxl5b + MO2-loxl1
Knockdown Reagents:	MO1-loxl1 MO1-loxl5b MO2-loxl1
Observed In:	notochord
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 307(2), Gansner, J.M., Mendelsohn, B.A., Hultman, K.A., Johnson, S.L., and Gitlin, J.D., Essential role of lysyl oxidases in notochord development, 202-213, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.