FIGURE

Fig. 6

ID

ZDB-FIG-070802-52

Publication

Gansner et al., 2007 - Essential role of lysyl oxidases in notochord development

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Fig. 6

Partial knockdown of loxl5b sensitizes embryos to notochord distortion in the presence of suboptimal copper nutrition (A–D) or disruption of col2a1 (E, F). Wild-type embryos were injected with 5 ng of control morpholino (A, C) or loxl5b morpholino (B, D), and incubated with (C, D) or without (A, B) 2 μM neocuproine starting at 3 hpf. Embryos injected with loxl5b morpholino develop notochord distortion (arrowhead) in the context of diminished copper availability (D). Embryos injected with 7.4 ng of col2a1 morpholino (E) are sensitized to develop notochord distortion (arrowhead) upon co-injection of 5 ng of loxl5b morpholino (F), demonstrating a genetic interaction between col2a1 and loxl5b. Photographs were obtained at 30 hpf (A–D) and 24 hpf (E, F).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-col2a1a WT + MO1-loxl5b WT + MO1-col2a1a + MO1-loxl5b
Knockdown Reagents:	MO1-col2a1a MO1-loxl5b
Observed In:	notochord
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 307(2), Gansner, J.M., Mendelsohn, B.A., Hultman, K.A., Johnson, S.L., and Gitlin, J.D., Essential role of lysyl oxidases in notochord development, 202-213, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.