Fig. 6

Fig. 6 Partial knockdown of loxl5b sensitizes embryos to notochord distortion in the presence of suboptimal copper nutrition (A–D) or disruption of col2a1 (E, F). Wild-type embryos were injected with 5 ng of control morpholino (A, C) or loxl5b morpholino (B, D), and incubated with (C, D) or without (A, B) 2 μM neocuproine starting at 3 hpf. Embryos injected with loxl5b morpholino develop notochord distortion (arrowhead) in the context of diminished copper availability (D). Embryos injected with 7.4 ng of col2a1 morpholino (E) are sensitized to develop notochord distortion (arrowhead) upon co-injection of 5 ng of loxl5b morpholino (F), demonstrating a genetic interaction between col2a1 and loxl5b. Photographs were obtained at 30 hpf (A–D) and 24 hpf (E, F).